Barca full-back Alba out of Spartak clash
There was better news for coach Tito Vilanova on Alexis Sanchez, who missed Barca's 4-win at Getafe on Sunday after returning from international duty with Chile with a minor leg muscle tear.
Although he has not been given the all-clear by medical staff, he was included in the 19-man squad for the match at the Nou Camp, Barca said on their website.
Barcelona must hit the ground running in Europe's elite club competition after sometimes labouring to progress in previous seasons, Vilanova told a news conference on Tuesday.
He takes charge of the favourites for the first time in the tournament having stepped up from assistant manager to succeed Pep Guardiola at the end of last season.
"In the Champions League you have to be on your guard from the start. We know this from experience because we have suffered in the past during the group stage," he said.
"The first match at home is very important so you don't start on the back foot. Barca have felt very comfortable in the Champions League in recent years but we know it's tough. The key thing is to take each game at a time."
Barca won the first and last of their four continental crowns at Wembley Stadium in London, at the old arena in 1992 and at the remodelled version in 2011, with triumphs in Paris in 2006 and Rome in 2009 in between.
Vilanova said it was too early to start thinking about winning a fifth at Wembley in May, when the stadium hosts its seventh European Cup final.
"If we have the good fortune to get to this stadium that holds such good memories for us then we talk about it," he said. "Right now it's too soon."
Barca are the only team in Spain to have won all four domestic league matches and Real Madrid have won one, drawn one and lost two, leaving them eight points behind.
However, Vilanova said a good start in La Liga had no bearing on the Champions League.
"There is no point in looking backwards, either when you are winning or when you are losing," Vilanova said.
"It's a different competition, I'll talk about La Liga again on Friday.
Group G is completed by Benfica and Celtic, who meet in Glasgow on Wednesday.