Pep Guardiola's side succesfully defended the cup with victory over Borussia Dortmund in May and will open their campaign for an 18th Pokal crown against Ralf Loose's men.
Loose is an ex-Borussia Dortmund player and his former employers will start their Pokal run against 3. Liga outfit Stuttgart Kickers.
The surprise package in the most recent Pokal, Kaiserslautern, begin with a trip to Wehen Wiesbaden of the third tier while fellow semi-finalists Wolfsburg make the trip to SV Darmstadt.
Four-time runners-up Duisburg host 2007 winners and recently-relegated Nuremberg, and Roger Schmidt's first Pokal campaign with Bayer Leverkusen starts with a trip to amateur side Alemannia Waldalgesheim.
Draw in full:
Homburg v Borussia Monchengladbach
Chemnitzer v Mainz
Optik Rathenow v St. Pauli
Eintracht Trier v Freiburg
SW Rehden v Aalen
Preussen Munster v Bayern Munich
Bremer v Eintracht Braunschweig
Holstein Kiel v 1860 Munchen
Bochum v VfB Stuttgart
Duisburg v Nuremberg
Viktoria Koln v Hertha Berlin
Stuttgarter Kickers v Borussia Dortmund
Alemannia Waldalgesheim v Bayer Leverkusen
Dynamo Dresden v Schalke
Magdeburg v Augsburg
Illertissen v Werder Bremen
Sieger Viktoria/Tasmania Berlin v Eintracht Frankfurt
RB Leipzig v Paderborn
Sportfreunde Siegen v FSV Frankfurt
Wehen Wiesbaden v Kaiserslautern
Darmstadt v Wolfsburg
Kickers Wurzburg v Fortuna Dusseldorf
Carl Zeiss Jena v Erzgebirge Aue
Paloma v Hoffenheim
Waldkirch v Greuther Furth
Braunschweig v Cologne
Energie Cottbus v Hamburg
Neubrandenburg v Karlsruher
Arminia Bielefeld v Sandhausen
Astoria Walldorf v Hannover
Heidenheim v Union Berlin
Kickers Offenbach v Ingolstadt
