Second-placed Real Madrid will be missing Spain central defender Raul Albiol for Saturday's match at city rivals Atletico Madrid after their appeal against his red card in last weekend's Getafe match was rejected.
Defenders Pepe and Ezequiel Garay returned to full training on Thursday and have been included in coach Manuel Pellegrini's squad, while midfielders Guti, Mahamadou Diarra and Royston Drenthe were omitted.
Forward Cristiano Ronaldo needs two more weeks to recover from an ankle injury.
Real's form has dipped in Ronaldo's absence but Pellegrini said he thought the team was on the right track and he was satisfied with Tuesday's 1-1 Champions League draw at AC Milan.
"Against Milan the team looked more like the one that we want to see," the Chilean told a news conference on Friday. "We maintained the pressure well and showed very good mobility."
Atletico's Argentina striker Sergio Aguero is feeling in good fettle ahead of the match at the Calderon after scoring both goals in Tuesday's Champions League draw against Chelsea.
"I am coming into the derby on good form," Aguero said on the club's website. Atletico are in 18th place with just one win in nine matches this term.
Pep Guardiola's Barcelona side did not train on Thursday after their long flight back from Kazan following Wednesday's 0-0 Champions League draw with Russian club Rubin was delayed by two hours.
Barca top the standings on 23 points, one ahead of Real and four ahead of Sevilla in third. Guardiola has a full squad available for Saturday's home match against fifth-placed Real Mallorca with the exception of long-term injury victim Gabriel Milito.
Guardiola said on Friday neither he nor the players were worried that Barca were not scoring as freely as last season.
"We have played teams that were very well structured defensively and we still managed to create a lot of chances," he told a news conference. "I am calm, the goals will come."
Mallorca will try to restrict the space and stop Barca from playing their customary brand of possession football, their coach Gregorio Manzano said.
"We have to put in some solid defensive work and try to prevent the match from opening up from the start," he said on the club's website. "We need to take our chances and use our ability to surprise them."
Sevilla midfielder Aldo Duscher picked up a muscle strain in Wednesday's 1-1 Champions League draw with VfB Stuttgart that put the club through to the knockout round with two matches to spare and will be out for several weeks, the club said.
Striker Frederic Kanoute trained as normal on Thursday and should be available for Sunday's match against visiting Villarreal, but defender Ivica Dragutinovic limped out of training and is doubtful, the club said on their website.
