Injuries have deprived Real Madrid of striker Karim Benzema and midfielders Guti and Royston Drenthe for Saturday's trip to second-from-bottom Tenerife while defender Alvaro Arbeloa is suspended.
Second-placed Real can go a point clear of Barcelona with a win at the Heliodoro. The leaders host improving Malaga in the day's late kick-off.
Real coach Manuel Pellegrini has warned his players against complacency.
"I hope we see a very focused Real Madrid, showing all the respect the opponent deserves," he told a news conference on Friday. "If we drop our level we'll suffer against Tenerife as other teams have done."
Injured Barcelona pair Seydou Keita and Eric Abidal were the only absentees from training on Friday and right back Daniel Alves appeared back to full fitness but had yet to receive the all-clear.
Coach Pep Guardiola has blamed himself for his side's recent patchy form which saw them suffer a first league defeat of the season at Atletico Madrid and a draw at VfB Stuttgart in Tuesday's Champions League last-16 first leg.
"We know we can do better because the whole world has seen very recently what we are capable of," he told a news conference. "The responsibility is exclusively mine."
Barca were boosted by the news central defender Gerard Pique agreed to extend his contract by three years to June 2015.
Spain international Pique is the 10th first-team player to sign a contract extension since the close season, following Yaya Toure, Victor Valdes, Pedro, Lionel Messi, Carles Puyol, Andres Iniesta, Rafael Marquez, Abidal and Jeffren.
Third-placed Valencia face a difficult trip to erratic Atletico Madrid on Sunday with both sides on a high after progressing to the last 16 of the Europa League.
Valencia midfielder Ever Banega said the team would be unaffected by fatigue after they won Thursday's second leg 3-0 against Club Bruges after extra-time for a 3-1 aggregate victory.
"There is hunger and attitude knowing we are in the Champions League qualification spots and for us it (fatigue) won't affect anything," he told a news conference.
Sevilla, who host seventh-placed Athletic Bilbao on Sunday, have winger Diego Capel back in contention after he missed Wednesday's Champions League draw at CSKA Moscow through injury.
"I am feeling good and ready to help the team," Capel told a news conference. The fourth-placed Andalucians are four points behind Valencia and four ahead of Deportivo La Coruna who visit Villarreal on Sunday.
