Atalanta eliminated by third-tier Lumezzane
Michele Marconi, on loan at Brescia-based Lumezzane from Bergamo's Atalanta, embarrassed his parent club with the only goal on 19 minutes.
Even the third division side had only two first-teamers in the line-up for the much-maligned competition and next travel to Udinese in the last 16 when the big Serie A clubs enter.
Palermo set up a meeting with holders Lazio after defeating Serie B side Reggina 4-1 at home in former Lazio boss Delio Rossi's first game since succeeding Walter Zenga as coach.
Napoli face Juventus next after beating second tier Cittadella 1-0 with the rest of the round completed next week.
On Wednesday, Serie A bottom side Siena suffered more pain in Alberto Malesani's first game in charge when they lost 2-0 at home to third division Novara, who now visit AC Milan, while Chievo progressed by overcoming Serie B's Frosinone 2-0.