Pablo Repetto's Independiente suffered a 2-0 loss at LDU Quito, marking their second straight loss in the league.
It opened the door for their nearest rivals and they took full advantage.
Barcelona, now just a point back, and Emelec both recorded victories to go with third-placed LDU Quito's win over the leaders.
Barcelona took advantage to move within a point, with Ismael Blanco's second-half goal enough to see them past Deportivo Quito 1-0.
Emelec are five points off top spot with three matches remaining after Miller Bolanos' late winner in their 1-0 victory at El Nacional.
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