Diego Simeone's charges fell victim to a 2-0 defeat at struggling Almeria last weekend, with a double from Jose Antonio Verza doing the damage, leaving Atletico level on points with Barcelona and Real Madrid at the top of La Liga.
Verza's second goal in that game came from the penalty spot, when goalkeeper Daniel Aranzubia was shown a straight red card for hauling down Jonathan Zongo.
Despite that run, Atletico's loss at Almeria was just their second in the league this term, and they had previously gone 13 league matches without defeat.
Furthermore, Simeone's side boast an undefeated record at the Vicente Calderon in the league this season, and with just one victory on the road this term Valladolid's chances of winning appear slim.
As well as doubts over Courtois, Atletico will be without midfielder Tiago (knee) and defender Javier Manquillo, who fractured a vertebrae in the Real defeat on Tuesday.
Despite those positive results, Juan Ignacio Martinez's charges remain mired in relegation trouble, and are three points adrift of safety in 18th position.
Their prospects have been hindered by an injury to forward Jeffren Suarez, who could miss over a month of action with a muscle injury.
