Leaders Juventus take their unbeaten record to Napoli on Sunday while struggling Inter Milan will attempt to bounce back from last weekend's loss at home to Juve when they visit Genoa.
Mihajlovic has been given the club's backing after he was jeered by a section of the crowd during last week's 1-0 win over Genoa, the hostile reaction including ethnic taunts against the former Yugoslavia international.
"The club cannot accept degrading behaviour, nor gratuitous vulgarity, aggravated by racism, and expresses its solidarity and support with Sinisa Mihajlovic, victim of shameful and intolerable attacks," Fiorentina said in a statement.
General manager Vincenzo Guerini added his own appeal: "I appeal to the Fiesole Curva to stop the jeering and offensive chanting against Mihajlovic.
"There was a sad atmosphere after the Genoa game, as if we had lost the match... those insults are not part of our history."
"We argue with the coach but we respect you as a man," said the banner. In return, Mihajlovic agreed to open up a practice match to the public.
However, it is his team's perceived lack of adventure which upsets the supporters as much as their results.
The 36-year-old has not started any of Juve's last six matches since being substituted at halftime in the match against Bologna, making only fleeting late appearances from the bench.
