Owen Coyle's side were beaten 1-0 at pool leaders Rubin Kazan in their last continental outing, but still sit a point clear of third-placed Zulte with just two games remaining.
A victory at the DW Stadium, coupled with a Rubin win over Maribor, would ensure Wigan's progress into the knock-out rounds.
Francky Dury's men have lost just one of their 16 league games this season, but have been beaten twice in the Europa League.
Wigan, meanwhile, have struggled to juggle league and cup form, and sit 11th in the Championship after Saturday's 1-0 defeat to Brighton.
"Bouncing back is the most important thing in football and we've got an opportunity to do that," he said.
"We've got another game on Thursday in the Europa League and it's at home so we have to prove to our fans that we can be much better.
"We're fortunate to have that opportunity and hopefully if we win it can be a boost for both our European and domestic chances."
Gary Caldwell is one of four players Coyle is set to be without, while the FA Cup winners also have concerns over Ivan Ramis (knee), Scott Carson (ankle) and Shaun Maloney (hip).
