Bayern are brimming with confidence, having carved out a 10-point lead over second-placed Bayer Leverkusen and 11 on Dortmund, in third.
The champions, who have yet to find the consistency this season that gave them back-to-back league titles, have already dropped two points this week with a 1-1 draw against promoted Fortuna Duesseldorf as Bayern won 2-0 at Freiburg.
A win for Dortmund on Saturday would see them remain in contention for the title but defeat would leave them 14 points adrift with Bayern showing no signs of slowing similar to last year's implosion at the same stage.
"After such unsuccessful games like against Fortuna we always come back with a show of defiance," said Dortmund defender Neven Subotic. "That is our trademark."
Apart from winning titles, they have also matched Bayern in Europe this season, joining them in the Champions League round of 16 as they challenge the Munich club's dominance.
"We should not make a crisis out of it," said Dortmund captain Sebastian Kehl after Tuesday's draw.
"We will be in different form on Saturday. We will try everything to win. The season is far from over but even us in Dortmund can see Bayern have opened up a big lead by playing very consistently."
"We are currently in very good shape," said Bayern midfielder Toni Kroos. "We have not been successful against Dortmund in the last few matches but this is something we want to change on Saturday."
