Warrington started the weekend in style, pulling off arguably the biggest shock of the first round when they defeated League Two side Exeter City 1-0 on Friday thanks to Craig Robinson's first-half header.
The Northern Premier League Division One North side, who were exactly 100 places below Exeter in the English league pyramid ahead of the match, are the lowest ranked team left in the competition and will have to pull off another shock in order to be in the hat with Premier League big boys such as Chelsea, Manchester United and FA Cup holders Arsenal.
Gateshead currently sit third in the Conference having lost just three times in the league this season and will look to make home advantage count at the Gateshead International Stadium.
Blyth Spartans will seek bragging rights when they travel to fellow north east team Hartlepool United, and with the latter currently bottom of League Two a potential upset could well be on the cards.
Non-league sides Chester FC and Dover beat League Two opposition in the shape of Southend United and Morecambe to reach round two, the former were handed a mouth-watering trip to Oakwell to face Barnsley, while the latter also hit the road to play Cheltenham Town.
Draw in full:
Oxford United v Tranmere Rovers
Bury v Luton Town
Scunthorpe United v Worcester City
Colchester United v Peterborough United
Hartlepool United v Blyth Spartans
Milton Keynes Dons v Chesterfield
Gateshead v Warrington Town
Cheltenham Town v Dover Athletic
