A drained Guardiola is to step aside and hand over the reins to his close friend and assistant Tito Vilanova for next season, but will hope to walk away with a 14th trophy from his glittering four-year spell in charge.
The former Spanish international kicked off his trophy-winning run with a 4-1 cup final win over Bilbao in May 2009.
"His departure is traumatic for me," Barca and Spain defender Gerard Pique, who returned to the Nou Camp just as Guardiola arrived in 2008, told a news conference.
"I feel I am a much better player thanks to him and the whole team has learned a great deal. He will leave a void, but we'll carry on with Tito and we'll do well. That's football, all eras come to an end."
They were denied a fourth consecutive La Liga crown by Real Madrid, and fell to Chelsea in the Champions League semi-finals, before Guardiola's shock decision not to renew his contract.
There have been criticism in some quarters at the way Vilanova's appointment was announced at the same news conference in which Guardiola said he was leaving, leading to speculation of a rift between the two.
"Guardiola's relationship with Vilanova is fantastic," midfielder Cesc Fabregas said earlier this week. "Hearing these comments from outside isn't good. It hurts us."
Basque and Catalan nationalists among the two sets of fans whistled the national anthem in 2009, and Esperanza Aguirre's suggestions on Tuesday that the final should be suspended if it happens again caused widespread condemnation.
"We need to be more relaxed from the start, not as nervous and as rushed with our passes [as we were in Bucharest]" Bilbao's leading scorer Fernando Llorente told reporters.
"We deserve to win this cup. We have managed to put behind us the disappointment of the other final. If we can play to our best and nullify Barca, we are capable of beating them."
