Pearce picked five forwards for the Euro 2012 warm-up with Wayne Rooney, Darren Bent, Daniel Sturridge, Danny Welbeck and Fraizer Campbell in contention for the starting places.
However, Rooney - who has 73 caps and 28 goals for England - pulled out with a throat infection on Sunday while Bent was prevented from picking up his 13th cap when he was withdrawn after suffering a serious ankle injury playing for Aston Villa.
Pearce, in charge initially just for Wednesday's match following Fabio Capello's resignation, decided against calling up any replacements such as Tottenham Hotspur striker Jermain Defoe and said he was happy with the three strikers he has left.
Asked if playing without an established international striker was a problem, Pearce replied: "We're in the home run for the championships in the summer and we have to find out about one or two individuals who have been around the squad and this is a great opportunity to find out.
"To play against one of the favourites for the tournament in front of a full house is a fantastic opportunity and there is no one in the squad that I would fear putting on the football pitch, they wouldn't be in the squad otherwise."
Bent could miss June's Euros, when England face France, Sweden and Ukraine, because of his injury while Rooney is suspended for the first two group games.
Liverpool defender Glen Johnson returned to his club later on Tuesday and Manchester City's Joleon Lescott was called up to replace him.
Pearce declined to reveal who would take over from John Terry, injured for the friendly but stripped of the captaincy by the FA because of an impending racial abuse trial which prompted Capello's departure earlier this month.
"It's a format I have followed at club level and at under-21 level and I don't see it necessary to change it and so that's where we are with the captaincy and the team selection," he said.
"I don't think they will learn a great deal, most of them probably weren't even born at that stage," he said.
"But I think we have to learn something from a side as good as the Dutch on Wednesday. We have to go into the game being confident and we have to come out of the game with a feel-good factor and a decent result.
"We are testing ourselves against one of the best sides in Europe."
