Lyon were without influential midfielders Juninho and Jeremy Toulalan and talismanic striker Karim Benzema, all of them rested, when they surrendered their trophy in a 3-2 defeat in the last-16 at Lille on Wednesday night.
The trio will return at the Stade de France as the leaders look to extend their unbeaten streak in Ligue 1 to 10 matches and defend a four-point lead (53 points from 26 games).
"It was quite hard to concede that goal in stoppage time but that's football. We must put this game behind us and focus on Saturday's game against the same team," Lyon midfielder Jean Makoun told the club's website.
"Both teams played the same match with the same effort. I hope Saturday's game will be as pleasing to watch, with a different result at the end," said Puel.
Second-placed Paris St Germain could reduce Lyon's lead to one point should they win at Lorient and the leaders lose.
"Twenty-four points away from home is a good performance but we definitely should have more from our home games," Marseille defender Laurent Bonnart told the club's website.
Saturday's crunch match features fourth-placed Girondins Bordeaux away to fifth-placed Toulouse, with the team that loses virtually out of the title race.
