Mancini told reporters that as far as he was concerned Tevez was "finished" and would never play for City again.
Mancini, looking visibly shocked, told a news conference: "I am really disappointed because it is Carlos.
"This can't happen in a top club - to refuse to go in to help his team mates. What I said to Carlos is between me, him and the team.
"But if we want to improve as team Carlos can't play with us. With me, he is finished."
Mancini said: "He refused to warm up and again refused to go on the pitch. If a player has a lot of money to play for Manchester City... he can't play, never."
He has only played one full match this season, in last week's win over Birmingham City in the League Cup.
He made a nine-minute appearance as a substitute in City's opening Champions League match two weeks ago against Napoli, and has also made two substitute appearances in the Premier League and started one game.
