The Italian was not booked in the match, which the Ukrainian side won 3-0, but was widely to reported to have elbowed Shakhtar captain Darijo Srna in the face.
De Rossi was suspended after a UEFA disciplinary hearing, a spokesman for European football's governing body said.
Shakhtar won 6-2 on aggregate to reach the quarter-finals, where they will meet competition favourites Barcelona.
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