John Terry is out of England's World Cup qualifier against Ukraine, the FA confirmed on Sunday.
The Chelsea defender picked up an ankle injury during England's Group H opener, a 5-0 win in Moldova on Friday.
"Following further treatment from the England medical staff today, John Terry will not be available," a statement on the FA's website read.
Terry had left the squad and returned to his club.
England host Euro 2012 co-hosts Ukraine at Wembley Stadium on Tuesday.