Dortmund have 62 points but have seen their impressive 12-point advantage shrink to just seven over second-placed Bayer Leverkusen following a loss and a draw in their last two fixtures.
Subotic's quick return from a foot injury sustained in Serbia's Euro 2012 qualifier against Northern Ireland last week, is a positive development for Dortmund, who are looking to maintain a healthy advantage at the top with seven games left.
"Neven's injury is painful but it also heals quickly. It should not be a problem for Saturday," coach Jurgen Klopp told reporters.
"Dortmund have taken a hit. Now we want to anger them a bit more and by doing that, we also want to strengthen our position," Hanover coach Mirko Slomka said.
"Ron told me he is still in pain. I am skeptical for the Dortmund game," Slomka added.
Next year's Champions League final will be held in Munich and the Bavarians are desperate not to miss out on a chance of making that final.
"I don't think I will ever be a bigger Dortmund fan than this weekend," Bayern president Uli Hoeness admitted, hoping for a Hanover defeat.
