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The Armenian, who was the subject of a €27.5million move from Shakhtar Donetsk early last week, picked up the problem during Dortmund's 4-1 friendly win over FC Luzern on Tuesday.
He scored his second goal in two pre-season appearances for the German club in that game, but limped from the field after sustaining the blow.
Mkhitaryan became Dortmund's most expensive signing of all time after finalising his move from Ukraine, with the player snubbing interest from Liverpool in order to join the 2012-13 UEFA Champions League finalists.
