He said Bayern's form in the Bundesliga had been patchy this season but noted that they had played extremely well in Europe's top club competition and had every chance of upsetting the Spanish league leaders.
"Obviously, we are all disappointed about Saturday's result but we know why it happened and we have cleared the air about it already," Klinsmann told a news conference. Bayern lost 5-1 away to VfL Wolfsburg.
"We have been impressive in the Champions League so far and that's what we are focusing on," he added.
"We have the quality and strength to live with Barcelona and also to win. We have proved that in previous games and people can expect a very exciting match."
Barcelona and Bayern are the joint-top scorers in this season's competition with 24 goals each but on Wednesday Bayern will be missing injured striker Miroslav Klose, who has netted seven in eight matches.
Klinsmann said losing Klose and central defender Lucio through injury had been a "very painful" blow and complained that the Germans had struggled with injuries the whole season.
"We will nonetheless make the best of it and adapt to the situation," he said.
"We have replacement players who are perfectly capable of challenging this Barcelona team. I am completely convinced of that. These body blows keep happening but we'll have to deal with it."
Asked about Barca's Argentina forward Lionel Messi, Klinsmann said he was the kind of player who could make the difference between victory and defeat.
"But we also have players who can make the difference," he added, singling out France midfielder Franck Ribery. "Tomorrow you'll be able to compare them and see who is the best."
