Second-placed Brentford suffered a comprehensive 3-0 defeat at Rotherham United, but third-placed Orient failed to take advantage as they were held to a 1-1 home draw with Oldham Athletic.
Kieran Agard scored a first-half double to put Rotherham in command, the striker's first coming from the penalty spot as he took his tally for the season to 20 goals.
Meanwhile, a late Korey Smith equaliser denied Orient all three points against Oldham after defender Scott Cuthbert had given Russell Slade's side the lead.
And Wolves took full advantage of their rivals' slip-ups, as they moved four points clear at the summit after coming out on the right side of a six goal thriller with Colchester United, Kenny Jackett's men claiming a 4-2 win.
Preston North End overcame Peterborough United 3-1 thanks to a late brace from Joe Garner, but MK Dons' play-off hopes were dealt a blow with a 1-0 reverse at home to Gillingham.
Elsewhere, Conor Coady scored twice as Sheffield United beat Crawley Town 2-0, and Craig Westcarr netted both goals in Walsall's 2-0 triumph at Bradford City.
