The Brazilian icon scored a sixth-minute penalty in Queretaro's 4-1 demolition of Guadalajara at the Estadio Omnilife on Sunday - ending the club's four-game winless streak.
After missing a penalty on his club bow in the Copa MX on Tuesday, Ronaldinho made no such mistake this time around - converting a spot-kick after former Mexico international Carlos Salcido was sent off for a foul inside the area.
Ronaldinho split Guadalajara's defence with an exquisite through ball between two centre-backs for Sanvezzo to poke the ball home in the 70th minute.
Giovanni Hernandez came off the bench and reduced the deficit with five minutes remaining, though substitute Othoniel Arce restored Queretaro's three-goal advantage in the fourth minute of injury time.
While America were 10-1 victors in the CONCACAF Champions League on Wednesday, Antonio Mohamed's men were three games without a win prior to kick-off against Pachuca.
But a Daniel Arreola own goal and Miguel Layun's strike 11 minutes from time secured maximum points for America.
However, Pachuca's joy was short-lived as Mexico international Layun popped up a minute later to win the match for America.
Defending champions Leon ended their three-game losing streak, Mauro Boselli's brace inspiring the hosts to a 3-1 triumph against fellow strugglers Veracruz.
