Adebayor was charged with improper conduct after running the length of the field to goad Arsenal fans when he scored against their club in the Premier League game won 4-2 by hosts City.
"We've drawn a line under that," Hughes told reporters on the eve of Wednesday's home quarter-final.
"It is a new game, probably against different personnel in terms of the team Arsenal are likely to pick, so I don't see it as an issue.
"Ade was very apologetic almost immediately and people possibly ignored that in the aftermath of what went on. He apologised, took his punishment as he had to, and we move on."
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