Mazemebe will still be smarting from conceding a late winner to Setif in the first leg away in Tunisia, especially since the game was played behind closed doors and they had several chances to snatch a result of their own.
However, the DR Congo side should be confident of overcoming the Algerians' 2-1 first-leg lead when they play them at home on Sunday.
However, Setif coach Kheireddine Madoui believes his side will take inspiration from the Algerian national side's superb 2014 World Cup run.
"The national team's excellent results in Brazil have had a positive impact on all Algerian clubs, none more so than Setif," he said.
"Watching them reach the last 16 for the first time has made our players are even hungrier for success. That's why we hope to achieve big things."
Vita Club should have put the tie to bed already after they wasted several clear-cut chances in the first leg.
The Congolese team looked to be missing influential striker Dady Birori who was banned from football for two years by CAF last week.
