Cole, England's first-choice left-back, broke his left ankle at Everton at the start of February and his return will be a boost for the west Londoners as they close in on a league and Cup double.
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"Ashley Cole is in the squad tomorrow," Ancelotti told reporters at Chelsea's training ground, an oasis of calm with the usual array of Bentleys and Ferraris in the car park.
"He is ready, he's fit, he's totally recovered from his injury and he will start from the bench tomorrow."
"He worked very well, also the medical staff worked very well on him," he said.
The manager added: "If he is not involved tomorrow he will play a friendly game next week but it is not necessary. He trained very well this week with the team and did a tackle without any problems. He is really fit 100 percent."
"The other players I don't know. I think that until 5.30 tomorrow I will choose the other eight. I prefer to sleep well tonight," he laughed.
"It's not a formality," he said of the league title. "We have to play some very difficult and important games and we have to pay attention. We know we have to fight."
Tottenham manager Harry Redknapp said: "We need to beat Chelsea to try to keep pushing for that fourth spot. After tomorrow we'll have a much clearer picture of what's going to happen. It's a tough run-in and they don't come any tougher than Chelsea.
"Chelsea are looking the strongest team. The atmosphere at White Hart Lane on Wednesday night was amazing and we need that again now. We need a lift to keep us going. We have got a lot of tired players at the moment."
