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Guillermo Barros Schelotto's Lanus side claimed a 2-1 victory at home to Santos Laguna in the first leg of their round-of-16 tie.
Carlos Quintero put the visiting Mexicans ahead just before the hour-mark, only for Argentina's Lanus to respond.
Quintero fired Pedro Caixinha's Santos Laguna into a 58th-minute lead, controlling a throw-in before smashing a finish into the roof of the net from a tight angle.
In Mexico, Leon came from behind to draw 2-2 at home to Bolivia's Bolivar.
Luis Montes and Juanmi Callejon traded first-half goals before the visitors went ahead through Juan Carlos Arce's 71st-minute goal.
