Football's world governing body also removed flags emblazoned with company logos during Sunday's Group D match between Ghana and Serbia at the Loftus Versfeld Stadium in Pretoria.
"In fact there were mass ambush activities by one company from Ghana during yesterday's match and as per the regulations the FIFA Rights Protection team had to collect a few flags carrying heavy commercial branding," FIFA spokesman Nicolas Maingot said in an emailed response to Reuters.
"Most other flags where the branding was only very small were not taken away."
The advertisement called Kulula the unofficial carrier of the "You-know-what" and showed footballs, vuvuzela fan trumpets and soccer players.
More than 60 percent of FIFA's 2007-2010 revenue of $3.2 billion comes from TV rights and about 30 percent from commercial marketing.
