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The Slovakian midfielder was introduced as a second-half substitute on Saturday as Napoli suffered a surprise defeat to Parma, but was forced off within 10 minutes after hurting his left foot.
Subsequent scans have shown Hamsik has avoided a fracture.
However, he will miss the trip to Signal Iduna Park, which will see Rafael Benitez's side seek a victory that would guarantee qualification for the knockout stages.
