Relegation-threatened Hull had shown signs of picking up a much-needed result in the first half, with Sone Aluko proving a constant nuisance to the Southampton back line.
However, Bruce lunged in on former Hull striker Shane Long to concede a 56th-minute spot-kick, and James Ward-Prowse – previously without a league goal to his name – made no mistake in finding the net.
James Chester came agonisingly close to restoring parity soon after when he saw a header cleared off the line by Ryan Bertrand, but Graziano Pelle made sure of maximum points with his first Southampton goal of 2015 nine minutes from time.
Wins for Burnley or QPR, who face Arsenal and Chelsea respectively this weekend, would see Hull slip into the bottom three.
Goalkeeper Allan McGregor was dropped to the bench following a disappointing performance in a 3-1 defeat at Swansea City last weekend and replacement Steve Harper – one of five changes for the visitors – soon came under pressure from Long, who was unable to make the most of a loose Jake Livermore backpass.
Harper went some way to justifying his selection by making himself big to keep out Pelle from close range after 20 minutes, while Aluko continued to give the home defence plenty to think about.
The Nigeria international's next effort swerved just wide from 25 yards, and Morgan Schneiderlin was lucky to escape a booking for a frustrated lunge on the Hull forward on the half-hour mark.
An advancing Aluko was then tugged back by Victor Wanyama, who was shown the yellow card and will miss Southampton's next two matches as a result.
Davis got down well to his left when Livermore - teed up by Aluko - fired a low drive at the bottom corner early in the second half, but an ill-judged Bruce challenge on Long in the box proved costly.
