There appears to be no stopping runaway Serie A leaders Juve in their quest to win a third consecutive Scudetto, as they hold an 11-point lead over second-placed Roma.
Fourth-placed Fiorentina are the only side to have beaten Antonio Conte's men in the Italian top flight this season when they won 4-2 at Artemio Franchi back in October, so Juve will have a score to settle.
This weekend's clash at Juventus Stadium also gives the two sides an opportunity to size each other up ahead of their UEFA Europa League round-of-16 first-leg clash at the same ground next Thursday.
Juve have had to settle for a place in Europe's secondary club competition after being dumped out of the UEFA Champions League and they will travel to Florence for the second leg on March 20.
Conte's immediate attention is on stretching the Turin giants' unbeaten run to 19 Serie A matches and moving a step closer to retaining their title.
He told Gazzetta dello Sport: "Fiorentina are a very strong side.
"They have a lot of ambition and they want to qualify for the Champions League next season.
"They are fourth in the standings and have already reached the Coppa Italia final.
"We will face them first in the league and then in the Europa League and we shall see how that goes."
Chiellini ultimately played no part in the 1-0 defeat against the world and European champions on Wednesday, but he could be back in the Juve side against Vincenzo Montella's charges.
