The Portuguese manager won the competition with Porto in 2010-11 as part of a treble in his first season in charge of the club.
It elevated Villas-Boas onto the world stage as he became the youngest manager to win a European title, at the age of just 33.
His attempts to bring the trophy to White Hart Lane were thwarted at the quarter-final stage last season, when Basel halted Tottenham's progress on penalties after the tie finished 4-4 on aggregate.
The London club only lost once in normal time in last year's Europa League - a 4-1 away defeat to Inter in another two-legged affair in which eight goals were shared, with a solitary away strike securing Tottenham's progress.
Etienne Capoue will miss the White Hart Lane match with the ankle injury that he sustained in the 1-0 loss to Arsenal, Tottenham's only defeat of the season, while fellow midfielder Nacer Chadli suffered a knock on international duty with Belgium and could also be absent.
But Agnar Christensen's men highlighted their ability to cause a shock when they beat league leaders Rosenborg on Sunday.
Midfielder Magnus Andersen's goal, his fourth of the campaign, proved decisive as Tromso ended their nine-match winless run in the league.
Remi Johansen carries Tromso's most potent goalscoring threat, with the striker having scored seven times in 20 league appearances this season.
