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Manager Antonio Conte named Marco Storari as Buffon's replacement for the match between the teams that top and tail Serie A.
"Tomorrow Storari will play because after the Inter match [in Serie A last weekend] Buffon suffered a debilitating attack of the flu," Conte said during Friday's pre-match news conference.
"After the [Champions League] match against Bayern [Munich on Tuesday] the flu came back, he wasn't training well and I would prefer that he took care of himself and got better.
Article continues below"Tomorrow there will be space for Storari, if I didn't have the guarantee of a good 12th man [substitute goalkeeper] I would have asked Gigi to play."
Champions Juventus are on 68 points, nine clear of second-placed Napoli.
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