"After a meeting (with directors) Eduardo Berizzo announced his time at Estudiantes was over and handed in his resignation," the club said on their website.
The team, winners of the Apertura championship in the first half of the 2010/11 season, are 15th in the Clausura with 19 points from 15 matches. The red and whites are 11 points off the pace after losing 2-0 at home to Independiente at the weekend.
The 2009 South American champions, with midfield general Juan Sebastian Veron missing through illness, were also knocked out of this year's Libertadores Cup in the first knockout round.
Berizzo is the seventh first division coach to have quit or been sacked during the Clausura after Miguel Angel Brindisi at Huracan, Leonardo Madelon at Quilmes, Fernando Gamboa at Colon, Roberto Sensini at Newell's Old Boys, Ramon Diaz at San Lorenzo and Angel Cappa at Gimnasia-La Plata.
