Sevilla winger Navas misses out having recently had surgery on an ankle injury.
The world and European champions face their neighbours at the Stadium of Light in Lisbon on Wednesday in a friendly they hope will boost the countries' joint bid to host the 2018 World Cup finals, coach Vicente del Bosque said on Friday.
"This is an important game being held just under a month away from the decision to choose the hosts for the 2018 World Cup finals," coach Vincente Del Bosque told a news conference on Friday.
"We can give the final push for the two countries to be successful in their bid."
Xavi, Cesc Fabregas, Fernando Torres, Pedro and Raul Albiol all return after missing last month's Euro 2012 qualifiers with injuries.
Midfielder Xavi, a candidate for the FIFA Ballon d'Or, has been struggling with an Achilles problem that has seen Barcelona using him sparingly this season, and led to him dropping out of October's internationals.
"Xavi has always shown his desire to come and play with the national team and if I remember correctly he is set to win his 97th cap," Del Bosque added.
"To doubt his commitment isn't correct."
Spain's last match of 2010 provides Portugal with the chance to avenge the 1-0 defeat they suffered in the World Cup finals, when David Villa scored the only goal to knock them out.
