Uruguayan forward Hector Gimenez scored twice in the last four minutes to give promoted Indios a 3-1 win over Guadalajara, ending their opponents' chances of qualifying and also putting them above America on goal difference in the overall standings.
They joined defending champions Toluca, Pachuca, Pumas UNAM, Monterrey, Puebla, Tecos UAG and Chiapas in the final eight of the tournament which has carried on despite the H1N1 flu outbreak.
The weekend's matches at San Luis, Atlas and Chiapas were played behind close doors while other games were subject to restrictions, which included limiting stadiums to 50 percent of their normal capacity.
Alberto Medina gave Guadalajara the lead just before halftime, putting them on course for a win which would have sent them through.
Daniel Frias fired the equaliser midway through the second half before Gimenez struck in the 87th minute and again in the first minute of injury-time.
The top two teams in each of the three first-round groups qualify along with the two best teams from the remainder, regardless of group.
Indios finished fourth in Group One with 23 points and a goal difference of minus one to scrape in as the second of the non-top two finishers. America were third in Group with 23 points and a goal difference on minus two.
Necaxa were relegated after losing 1-0 at America on Saturday while Atlas, under former Mexico coach Ricardo La Volpe, blew their chances when they were thumped 5-0 at home by Pachuca.
