Rosicky went to hospital for a scan on his Achilles on Wednesday along with keeper Petr Cech who has a sore shoulder. They have been given a 75 percent chance of being fit.
Coach Michal Bilek has therefore been left to sweat over whether they can shake off their injury problems for the Czechs final Group A match against co-hosts Poland on Saturday.
"We wanted to attack even more but it was difficult when Rosicky left," Bilek said. "He is our key player."
Russia's 1-1 draw with Poland kept them top of the group with four points ahead of the Czech Republic on three and Poland with two while Greece are bottom with just one point.
But the Czechs will be wary of a Polish side that showed it was possible to soak up Russia's early attacks and create their own chances, something Bilek's side spectacularly failed to do in their opening 4-1 loss to the group favourites.
Young midfielder Vladimir Darida, a last-minute addition to the squad due to Rosicky's injury worries, is another option.
"It is [always] a great loss to not have Tomas Rosicky on the pitch," said Vaclav Pilar, who has scored a goal in each game. "He is our key player."
