Monday's mauling in the 'Clasico' was Real's first loss of the season and the margin and manner of it surprised everyone as they slipped off the top of La Liga down to second, two behind points their arch-rivals.
Coach Jose Mourinho was given a one-match Champions League ban and four players were fined by UEFA on Tuesday over allegations that Sergio Ramos and Xabi Alonso deliberately provoked red cards in a match against Ajax last month.
"Our pride in the shirt and the club's badge has been hurt, especially as we know many Madrid fans are still suffering from the (Barca) defeat," Real and Spain defender Ramos told reporters.
"We need to pick ourselves up and stop feeling sorry for ourselves. Not to do so would be a mistake. On Saturday we have an important game against rivals who are having a good season."
Games between Real and Valencia have traditionally been high-octane affairs, and Unai Emery's side arrive in good form and unbeaten in five.
"Real will be intense and keen to win to help them forget about the other day's game," Valencia's Spain winger Juan Mata said. "I hope we don't catch a backlash."
"They won't have it so easy here. They know what to expect," Osasuna midfielder Fernando Soriano said.
"We need to be committed and make sure they know about it. It needs to be a physical game and very tough."
