Arsenal will be without inspirational captain Cesc Fabregas, who completed their unexpected comeback with a late penalty to level 2-2 against Barca in London last week but cracked a bone in his leg in the process.
They will also be missing injured William Gallas, forward Andrei Arshavin and midfielder Alex Song, as they seek a win or a high-scoring draw to advance to the last four.
"I know that they will fight for it," Arsenal coach Arsene Wenger told a news conference on Monday. "I always supported this team even in the weak points of the season because I believe they have some exceptional mental strength."
Holders Barca, whose opening 20 minutes in the first leg were described as a "storm" by Wenger, have their own injury and suspension concerns.
In Tuesday's other quarter-final, CSKA Moscow will be hoping their artificial pitch will provide a much-needed advantage to overcome a 1-0 deficit against dominant Inter Milan from the first leg and become the first Russian side to reach the Champions League semi-finals.
Mourinho's options also include 19-year-old forward Mario Balotelli, who scored on Saturday after spending a month on the sidelines following a row with the Portuguese manager.
