Banfield, who rested most of their first team players ahead of their opening South American Libertadores Cup match at home to Mexico's Morelia on Wednesday, went ahead through striker Cristian Garcia after 10 minutes.
Chacarita hit back with goals from defender Lisandro Lopez, namesake of the Argentina striker who plays for Olympique Lyon, and Facundo Parra either side of halftime to boost their relegation average.
Striker Mauro Boselli and captain Juan Sebastian Veron, with a brilliant effort, scored first half goals to give Libertadores Cup holders Estudiantes a 2-0 home win over San Lorenzo.
Estudiantes, who were at full strength, open their defence of the South American title at home to Juan Aurich of Peru in La Plata on Thursday.
River Plate, without former Argentina forward Ariel Ortega who was dropped for arriving late for their match, were held 0-0 by Rosario Central at their Monumental stadium. They have taken four points from three matches.
BOCA BEATEN
Their arch-rivals Boca Juniors went three goals down within 18 minutes as they lost 4-2 at Newell's Old Boys in Rosario on Saturday.
Boca, whose coach Alfio Basile resigned less than two weeks before the tournament started, picked up four points from their opening two matches but their ageing defence has now shipped seven goals in three games.
Strikers Martin Palermo and Lucas Viatri pulled two goals back before 10-man Newell's, who had defender Agustin Alayes sent off with 23 minutes to go, scored a fourth through former Monaco midfielder Lucas Bernardi in stoppage time.
In the pick of Saturday's matches, Argentinos Juniors came from two down to crush Lanus 6-3 with two goals by striker Nicolas Pavlovich.
Velez Sarsfield, winners of last year's Clausura in July, are top with seven points from three matches after Saturday's 2-1 home win over Gimnasia-La Plata.
