Poland international Lewandowski arrived at the Bundesliga giants from Lech Poznan in 2010 and has established himself as one of the best strikers in the world during his time at Dortmund.
The 25-year-old - who has scored 72 Bundesliga goals during his time at the club - will now bid farewell to the home supporters when Dortmund host Markus Gisdol's men, ahead of his free transfer to rivals and Bundesliga champions Bayern Munich.
Dortmund head coach Klopp harbours no ill-feeling towards the forward however, and says he leaves the club a "world-class" striker.
"He arrived as a great striker and leaves as a world-class striker," Klopp said at his pre-match press conference.
Lewandowski will play for Dortmund twice after Saturday's encounter, firstly in the club's last Bundesliga fixture at Hertha Berlin before he faces his future employers in the May 17 final of the DFB-Pokal.
"Lewandowski has been a brilliant striker for us and it's been a great time," Klopp added. "I hope he gives it everything in his last 3 games."
Dortmund head into the encounter protecting a seven-match unbeaten run in Germany's top flight, and their fine run has left Klopp's charges wondering what might have been had they not suffered such a horrendous injury crisis in defence this season.
Dortmund's recent upturn in form is sure to be tested by a Hoffenheim side that have performed admirably in the Bundesliga this season.
Gisdol's men have had a far more comfortable campaign this time around and, with just two games remaining, appear set to clinch a top-10 finish.
