Peter Bosz's Vitesse side led the Eredivisie from mid-November until their 1-1 draw away to NEC Nijmegen last week saw Ajax move top of the Dutch top tier with their 1-0 triumph on the road at Go Ahead Eagles.
Vitesse (41 points) are in danger of dropping further behind Ajax (43) when they travel to Rotterdam to play Feyenoord (36), who sit fourth in the league.
The Arnhem-based club have not won at Feyenoord since 2005 and while Friday's hosts lost 3-2 away to struggling ADO Den Haag last week, they have won all but three home matches this season, including their past three.
The past three matches have seen Vitesse stutter with Bosz's side giving up a lead each time to draw twice and win once.
Vitesse's young midfield trio of Davy Propper, Marko Vejinovic and Christian Atsu will be crucial to their side's hopes having stepped up in the absence of Theo Janssen, who is out for the rest of the season after tearing the anterior cruciate ligament in his left knee in October.
Ajax cruised past Jan Wouters' men 3-0 earlier in the season and should be confident up against a side that have lost their past four games, although Utrecht are sure to be up for the contest against the Amsterdam giants.
Twente could overhaul Vitesse if they lose to Feyenoord with the Enschede-based club four points behind Bosz's men with a game in hand.
If Twente beat Groningen on Wednesday in their left over match and defeat Cambuur on Sunday, they will move to 43 points, which would see them level with Ajax if Frank de Boer's team slip up against Utrecht.
