The former Benfica striker was a prolific goalscorer for both club and country - scoring 733 goals in 745 matches - and boasts an impressive list of honours to his name.
Here we look back at the greatest achievements of a storied career that featured 11 league titles and one European Cup win:
1961: Wins first Portugal cap in a 4-2 defeat to Luxembourg, scoring his country's opening goal
1962: Scores two goals in European Cup final as Benfica beat Real Madrid 5-3, finishes second behind Josef Masopust of Dukla Prague in Ballon d'Or voting.
1963: Suffers defeat in 2-1 European Cup final loss to Milan, scoring Benfica's only goal, but wins second league title with Lisbon club.
1965: Wins Ballon d'Or ahead of Inter duo Giacinto Facchetti and Luis Suarez.
1966: Loses World Cup semi-final to hosts England, despite netting Portugal's only goal, Bobby Charlton scoring a double for the eventual winners. Finishes tournament as top scorer (nine goals).
1968: Prevented from scoring winner in European Cup final by great stop from Manchester United goalkeeper Alex Stepney with scores at 1-1. United went on to win 4-1 in extra-time, but Eusebio displayed great sportsmanship by applauding Stepney's save.
1979: Retires as Benfica's all-time leading goalscorer 474, a mark that still stands. Also finished his career as Portugal's all-time leading goalscorer (41 goals) and most capped player (64 caps), although both records went on to be broken.
2003: Honoured as the Golden Player of Portugal by the Portuguese Football Federation to celebrate UEFA's jubilee.
