The duo are currently level on points at the summit of the Spanish top flight - Barca leading the way on goal difference - and are yet to meet in the league this season.
They did, however, contest the Supercopa de Espana in August, with Barcelona emerging as champions on away goals after a 1-1 draw at the Vicente Calderon was followed by a goalless affair at Camp Nou.
Atletico have won just one of the last eight league clashes between the sides, scoring just eight goals compared to Barca's 24.
Barcelona could be boosted by the league return of Lionel Messi, who scored twice after coming on as a second-half substitute in Wednesday's 4-0 Copa del Rey win over Getafe following a two-month spell on the sidelines with a hamstring injury.
Real Madrid will be keen spectators of Saturday's clash at the Vicente Calderon as they prepare to visit Espanyol in the weekend's other Madrid-Barcelona clash on Sunday.
Carlo Ancelotti's men currently sit five points adrift of the leading pair, but are on an eight-match unbeaten run in the league.
Basement club Real Betis' fight for survival continues as they welcome Osasuna to Benito Villamarin, and the club will be looking for a first win in 12 La Liga fixtures.
