Serie A champions Juve overcame the disappointment of suffering a first league defeat since March at Genoa last month by bouncing back with back-to-back victories.
Massimiliano Allegri's side claimed a routine 2-0 win at Empoli before thrashing Parma 7-0 last time out.
"Is the challenge of Olimpico a trap? Yes it's true, and then after the international break it's always very difficult, regardless of your opponents," Marchisio told Il Messaggero.
"You never know how to return from international duty.
"It will be a tough away match, Lazio can hurt us. We will find ourselves in a tough environment, against a great team who are tough, competitive, well-trained and are doing very well.
"We aren't just going on a trip, we want the three points."
Lazio will undoubtedly hope that Juve have one eye on next week's crucial UEFA Champions League meeting with Malmo, with the Turin's club's hopes of reaching the last 16 of Europe's premier club competition hanging in the balance.
And while forward Filip Djordjevic knows they face a tough task this weekend, he is keen to continue that impressive run.
"They [Juventus' players] are all quality players," he told Sky Sport Italia. "They are the best in Italy and we are motivated to do well.
"It is a tough game, but we want to win because we are at home."
Lazio coach Stefano Pioli, meanwhile, is boosted by the return of Stefan Radu.
