Goalkeeper Gianluigi Buffon, defensive trio Andrea Barzagli, Giorgio Chiellini (pictured with Buffon) and Leonardo Bonucci and midfielders Andrea Pirlo and Claudio Marchisio are all from the Turin club.
The Italians have already qualified from Group C with two matches to spare and Prandelli has decided to field an adventurous 4-4-2 formation.
"The match with Serbia should be a good barometer of our credentials because they are technically one of the best teams in Europe," Prandelli told a news conference on Thursday.
"We will be fully motivated because there is strong competition in our squad for spots in the starting line-up and this game is the start of our preparations for the finals.
"Giuseppe Rossi has shaken off his injury, he went the distance in a very intense training session, so he will start up front alongside Antonio Cassano," added Prandelli.
"The Italians are lethal on the break and we have to be smart and patient in order to avoid falling behind because that would make life very difficult for us," Petrovic said.
"We will be in touch with what's going on in Belfast because the outcome of that match is very important for us but the priority is to stay completely focused on our own task."
The winners of Europe's nine qualifying groups and the best second-placed team gain automatic berths in the finals alongside joint hosts Poland and Ukraine while the other eight runners-up enter two-legged play-offs for the remaining four places.
