The strikers picked up muscle injuries during Sunday's warm-up clash with Algeria and are reported to be facing a month on the sidelines.
With that in mind, coach Georges Leekens has handed to call-ups to Edem Rjaibi and Ahmed Akaichi to replace the pair.
CA Bizertin's Rjaibi has been involved in the senior squad previously, but is yet to make his debut, while ES Tunis forward Akaichi was part of Tunisia's 23-man party at the 2010 tournament.
Despite the deadline for naming squads having passed, countries are able to replace injured players before their first match.
Tunisia kick off their campaign against Cape Verde on Tuesday and also face Zambia and DR Congo in Group B.
Watch every match of the Africa Cup of Nations LIVE on British Eurosport HD or sign up to Eurosport Player and follow the action on mobile, tablet and other devices: www.eurosportplayer.com
Get the best features, fun and footballing frolics straight to your inbox every week.
Thank you for signing up to Four Four Two. You will receive a verification email shortly.
There was a problem. Please refresh the page and try again.