"We are very strong at home and it will be a different game back in London," Di Matteo told reporters after the holders went down 2-1 at the Donbass Arena.
Chelsea host Shakhtar at Stamford Bridge in their next Champions League fixture on November 7 and the former Italy midfielder knows his side must improve if they are to qualify for the knockout stages.
"We have to win our remaining two home games, that's for sure," he said, also referring to the match against Danish underdogs Nordsjaelland on December 5.
"It is very difficult when you concede an early goal," said Di Matteo. "It's a shame we got back into the game a bit late.
"I believed we could score at least but they punished us after we lost the ball in midfield for the second goal. We have to do better as a team in those situations."
"Our supporters saw the real Shakhtar tonight," said defender Darijo Srna. "We looked much better than Chelsea in this game and it is a pity we did not manage to score more goals.
"Our players have grown in confidence this season so this victory was not accidental. It's a great win for us."
