Lyon will win Group F with a victory or a goalless draw. Any other result would hand first place to Bayern.
The seven-times French champions made sure of advancing with a 2-1 win at Fiorentina in which the Brazilian pair of midfield inspiration Juninho and defender Cris collected a yellow card each meaning they will be suspended on Wednesday.
Their absence is a problem for coach Claude Puel, already missing defenders Francois Clerc, Anthony Reveillere (pictured) and John Mensah through injuries.
Puel could also have to make do without his most dangerous weapon, France striker Karim Benzema, a doubtful starter because of a sore thigh muscle.
Bayern coach Juergen Klinsmann has the enviable task of picking a starting lineup from a healthy squad that includes Hamit Altintop who returned to action for the reserve team at the weekend after four months out to mend a broken metatarsal.
Buoyed by their 2-1 home win against league leaders Hoffenheim, the German champions will be hoping to go into the winter break in style, capping the group stage with a win before travelling to Stuttgart for their last league match of the year.
Luca Toni and Miroslav Klose should start up front with Lukas Podolski recovering from a bout of flu.
Lyon, desperate to make an impact in Europe at last after never advancing past the quarter-finals of the Champions League, warmed up for Wednesday's match by losing 2-1 at strugglers Nantes in Ligue 1 on Saturday.
That defeat left them with just one point from their last three league outings and under threat from Olympique Marseille, three points back in second place before visiting Lyon next Sunday.
"One point from three games is really worrying but we only have ourselves to blame for it," said Lyon midfielder Jeremy Toulalan. "The Champions League, however, is a different story. It's time for us to turn the page."
Probable teams:
Olympique Lyon: 1-Hugo Lloris; 32-Lamine Gassama, 28-Jeremy Toulalan, 4-Jean-Alain Boumsong, 11-Fabio Grosso; 26-Fabio Santos, 17-Jean Makoun, 6-Kim Kallstrom; 14-Sidney Govou, 7-Ederson; 9-Fred.
Bayern Munich: 1-Michael Rensing; 23-Massimo Oddo, 3-Lucio, 5-Daniel van Buyten, 21-Philipp Lahm; 31-Bastian Schweinsteiger, 17-Mark van Bommel, 7-Franck Ribery, 15-Ze Roberto; 9-Luca Toni, 18-Miroslav Klose.
Referee: Howard Webb (England)
