Agbonlahor in for final two qualifiers
England, unbeaten in their group and through to next year's tournament in South Africa with two games to spare, travel to Ukraine on October 10 and host Belarus four days later.
Portsmouth goalkeeper David James returned after injury for the first time since April in place of Manchester United's Ben Foster.
In-form Aston Villa striker Gabriel Agbonlahor replaced Tottenham Hotspur's Jermain Defoe who has a hand injury.
Attacking midfielders Joe Cole and Theo Walcott, both recently returned from lengthy spells on the sidelines at Chelsea and Arsenal, missed out.
Goalkeepers: Robert Green (West Ham United), David James (Portsmouth), Paul Robinson (Blackburn Rovers).
Defenders: Wayne Bridge (Manchester City), Wes Brown (Manchester United), Ashley Cole (Chelsea), Rio Ferdinand (Manchester United), Glen Johnson (Liverpool), Joleon Lescott (Manchester City), John Terry (Chelsea), Matthew Upson (West Ham United).
Midfielders: Gareth Barry (Manchester City), David Beckham (LA Galaxy), Michael Carrick (Manchester United), Steven Gerrard (Liverpool), Frank Lampard (Chelsea), Aaron Lennon (Tottenham Hotspur), James Milner (Aston Villa), Shaun Wright-Phillips (Manchester City).
Forwards: Gabriel Agbonlahor (Aston Villa), Carlton Cole (West Ham United), Peter Crouch (Tottenham Hotspur), Emile Heskey (Aston Villa), Wayne Rooney (Manchester United).