Thiago Silva has not been named in Paris Saint-Germain's 18-man squad to face Barcelona at Parc des Princes in the Champions League after failing to recover from a calf injury.
Brazil centre-back Silva missed last Friday's 3-0 win at Bordeaux due to the complaint and has failed to recover in time.
It means 21-year-old defender Presnel Kimpembe is in line to start against Barca's all-star forward line of Lionel Messi, Luis Suarez and Neymar in Tuesday's first leg of a keenly anticipated last-16 tie.
Kimpembe has made 16 appearances for Unai Emery's side in all competitions this season but is yet to feature in the Champions League.
Before PSG take on tomorrow, see how they advanced to the Round of 16 February 13, 2017
Speaking at his pre-match news conference, with speculation rife over Silva's injury but before his absence was confirmed, Barca boss Luis Enrique acknowledged the 32-year-old's importance to the PSG cause.
"That's something that I just learnt about, that Thiago Silva may not be able to play," he said.
"He is one of the pillars of the team. Personally, I like the best players to play. That will make the show all the better."
